Journal: AMB Express

Article Title: Real-time loop-mediated isothermal amplification: an early-warning tool for quarantine plant pathogen detection

doi: 10.1186/s13568-019-0774-9

Figure Lengend Snippet: Comparison of different DNA extraction and LAMP protocols for Xylella fastidiosa , Ceratocystis platani and Phytophthora ramorum detection

Article Snippet: As negative control, fresh tissue collected from 10 healthy plant of each tested species ( Olea europaea , Rhamnus alaternus , Calicotome spinosa , Cistus incanus , S. junceum , Prunus dulcis , Platanus × acerifolia and Viburnum tinus ) were extracted by using both Plant Material DNA extraction kit (OptiGene) and EZNA Plant DNA Kit (Omega Bio-tek), as previously described.

Techniques: Comparison, DNA Extraction, Lysis, DNA Amplification, Amplification, Stripping Membranes, Electrophoresis

Journal: Developmental cell

Article Title: Potently cytotoxic natural killer cells initially emerge from erythro-myeloid progenitors during mammalian development

doi: 10.1016/j.devcel.2020.02.016

Figure Lengend Snippet:

Article Snippet: For murine samples, RNA was isolated using the Qiagen RNeasy Kit (Qiagen) and transcribed into cDNA using the iScript cDNA synthesis kit (BioRad).

Techniques: Purification, Recombinant, Modification, Knock-Out, Staining, Isolation, Expressing, Lysis, Software

Journal: Biodrugs

Article Title: Immunogenicity of Gene and Cell Therapies

doi: 10.1007/s40259-025-00756-8

Figure Lengend Snippet: Factors influencing immunogenicity to adeno-associated viral vector (AAV) gene therapy and chimeric antigen (CAR) T-cell therapy. General structures of recombinant AAV, transgenic expressed protein, and fourth-generation CAR T cells are shown. Factors shown to influence propagation of immune response to each are listed below respectively. CDR complementary-determining region, PTM post-translational modifications, PTP previously treated patients, PUP previously untreated patients, sc self-complementary, scFv single-chain fragment variable, ss single-stranded. Figure was created with BioRender.com

Article Snippet: Clinical data from anti-CD19 CAR T therapies having murine scFv regions, such as Yescarta (CD28 based) and Kymriah (4-1BB based), indicate that although a small fraction of patients develop de novo anti-FMC63-binding antibodies, these antibodies generally did not affect initial expansion or early persistence of CAR T. This suggests that while humoral responses can be detected, their early impact on CAR T kinetics and efficacy may be limited by factors such as lymphodepletion and rapid CAR T expansion [ , ].

Techniques: Immunopeptidomics, Plasmid Preparation, Recombinant, Transgenic Assay